Zorbax Rx/Rx-Sil
Zorbax Rx/Rx-Sil HPLC Columns from Agilent Technologies
ZORBAX Rx columns are made from pure porous silica microspheres and are optimized for high throughput analysis. Rx-C18 is recommended for alternate selectivity at low pH relative to Eclipse XDB-C18 and StableBond SB-C18. With low acidity and metal content, Rx-SIL is ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica. The Rx-SIL is also stronger than other silica types and is less acidic than the ZORBAX-SIL.
Rx columns are available in 1.8, 3.5 and 5 µm particle sizes for high speed, high resolution and analytical separations.
All pictures shown are for illustration purpose only. Actual product may vary.
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- Features
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- Made from highly pure (> 99.995 %) porous silica microspheres
- Available in 1.8 and 5 µm particle sizes
- Stronger than older silica types
- Less acidic than ZORBAX SIL, lower metal content
- Low acidity and low metal content make Rx-SIL ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica
- Rx-SIL useful for very hydrophilic compounds with high organic mobile phases in HILIC mode
- Rx-C18 recommended for alternate selectivity at low pH relative to Eclipse Plus C18, Eclipse XDB-C18 and StableBond SB-C18; for higher temperature applications, StableBond is recommended
- Rx-C18 higher carbon load than SB-C18 columns (12 % versus 10 %)
- High stability and good peak shape for low pH applications (up to pH 8)
- Specifications
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Pore Size Surface Area Temp. Limits pH Range* End capped Carbon load USP Designation ZORBAX Rx-C18 80 Å 180 m²/g 60 °C 2 - 8 No 12% L1 ZORBAX Rx-C8 80 Å 180 m²/g 80 °C 1 - 8 No 5.5% L7 ZORBAX Rx-SIL 80 Å 180 m²/g - 0 - 8 No - L3 *ZORBAX Rx columns are designed for high stability at low pH (e.g. pH < 5). However, all silica-based packings have some solubility in pH > 6 aqueous mobile phases. Therefore when using silica-based columns under conditions of pH > 6, maximum column lifetime is obtained by operation at low temperatures (< 40 °C) using low buffer concentrations in the range of 0.01-0.02 M. Column stability at pH>6 is also enhanced by avoiding phosphate and carbonate buffers.
- Literature